Journal: Immunity

Article Title: Tissue resident iNKT17 cells facilitate cancer cell extravasation in liver metastasis via interleukin-22

doi: 10.1016/j.immuni.2022.12.014

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Article Snippet: A second PCR product using the pGEM-T Easy-IL-22 construct template with Phusion Hot Start Flex DNA polymerase and primer set B was performed that was sub-cloned into pCMV-EGFP C1 (Clontech) mammalian expression vector by the InFusion ligation system (Takara).

Techniques: Recombinant, Clone Assay, Plasmid Preparation, Cell Adhesion Assay, Inverted Microscopy, Microscopy, Infection, Software

Journal: Immunity

Article Title: Tissue resident iNKT17 cells facilitate cancer cell extravasation in liver metastasis via interleukin-22

doi: 10.1016/j.immuni.2022.12.014

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Article Snippet: Phusion® Hot Start Flex DNA polymerase , New England Biolabs , Cat#M0535S.

Techniques: Control, Virus, Recombinant, Reverse Transcription, Clone Assay, Plasmid Preparation, Cell Adhesion Assay, Inverted Microscopy, Microscopy, Infection, Software

Journal: Antiviral research

Article Title: Raltegravir blocks the infectivity of red-fluorescent-protein (mCherry)-labeled HIV-1 JR-FL in the setting of post-exposure prophylaxis in NOD/SCID/Jak3 −/− mice transplanted with human PBMCs

doi: 10.1016/j.antiviral.2017.09.003

Figure Lengend Snippet: For comparison, the largest lymph node in each mouse was examined. The largest lymph nodes in each mouse less than 0.01 mg were expressed as 0 mg in Panel A. For comparison of the numbers of lymph nodes, mC signal-emitting lymph nodes were counted using the Maestro™ system as shown in Panel B. Statistical analyses were performed using a nonparametric multiple comparison called Bonferroni method.

Article Snippet: Statistical analyses Nonparametric statistical analyses were performed by using the Mann-Whitney U test (Statview, version 5.0; Abacus Concepts) and Bonferroni method (Stat Flex, 5.0 software; Artec).

Techniques: